Science Forum

maebelle · Posted: Mon Jun 23, 2008 12:49 pm Post subject:

I'm doing an experiment where I must add frozen Vitamin E powder to methanol, and then add that solution to PBS. I think there must be an easier way of getting solid Vitamin E into solution than the method my PI has taught me.

He has me add methanol to Vit E. to create a stock solution, and then add the desired amount into the PBS + media solution by pipette, while vortexing the conical tube with the media in it, if that makes any sense. This supposedly makes sure the vitamin E goes in the tube, and doesn't stay in the pipette tip.

Does anyone have a better way to do this, or thinks the vortexing is just adding an unnecessary step?